Isolation and partial characterization of drug-resistantAedes albopictus cells
- 1 March 1978
- journal article
- Published by Springer Nature in Somatic Cell and Molecular Genetics
- Vol. 4 (2), 179-191
- https://doi.org/10.1007/bf01538983
Abstract
We report here the first isolation and characterization of drug-resistant cell lines from cultured mosquito cells. A BrdU-resistant cell line was isolated from mutagen-treatedAedes albopictus cells, and then used as the parent cell from which sublines resistant to ouabain or α-amanitin were derived. The BrdU-resistant cell line and all sublines derived from it were shown to be deficient in thymidine kinase. Evidence that favored attributing the drug-resistant phenotypes to mutant genotypes included (a) the drug-resistant phenotypes were stable for up to 16 months of culture under nonselective conditions, and (b) the frequency of the ouabain-resistant cells in a sensitive population was increased 500-fold (to between 10 −3 and 10 −4 ) by exposure to the chemical mutagen EMS. Evidence illustrating the usefulness of mutant mosquito cells for the study of the genetics of somatic cells is discussed.Keywords
This publication has 15 references indexed in Scilit:
- On the nature of hereditable variation in cultured somatic cellsCell, 1976
- α-amanitin resistance: a dominant mutation in CHO cellsCell, 1975
- A simple cytochemical technique for demonstration of DNA in cells infected with mycoplasmas and virusesNature, 1975
- Ouabain-resistant mutants of mouse and hamster cells in cultureCell, 1974
- A mutational assay system using the thymidine kinase locus in mouse lymphoma cellsMutation Research, 1972
- Infection of Thymidine Kinase-Deficient BHK Cells with Polyoma VirusNature, 1966
- HeLa cells resistant to bromodeoxyuridine and deficient in thymidine kinase activityInternational Journal of Cancer, 1966
- Induction of thymidine kinase by vaccinia-infected mouse fibroblastsJournal of Molecular Biology, 1963
- Properties of L cells resistant to 5-bromodeoxyuridineExperimental Cell Research, 1962
- Amino Acid Metabolism in Mammalian Cell CulturesScience, 1959