Human basement membrane collagen (type IV)
- 1 February 1988
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 172 (1), 35-42
- https://doi.org/10.1111/j.1432-1033.1988.tb13852.x
Abstract
The cDNA and protein sequences of the N-terminal 60% of the .alpha.2(IV) chain of human basement membrane collagen have been determined. By repeated primer extension with synthetic oligodeoxynucleotides and mRNA from either HT1080 cells or human placenta overlapping clones were obtained which cover 3414 bp. The derived protein sequence allows for the first time a comparison and alignment of both .alpha. chains of type IV collagen from the N terminus. This alignment reveals an additional 43 amino acid residues in the .alpha.2(IV) chain as compared to the .alpha.1(IV) chain. 21 of these additional residues form a disulfide-bridged loop within the triple helix which is unique among all known collagens.This publication has 43 references indexed in Scilit:
- Construction of a model for the aggregation and cross‐linking region (7S domain) of type IV collagen based upon an evaluation of the primary structure of the α1 and α2 chains in this regionEuropean Journal of Biochemistry, 1987
- Nucleotide sequence coding for the human type IV collagen α2 chain cDNA reveals extensive homology with the NC‐1 domain of α1 (IV) but not with the collagenous domain or 3untranslated regionFEBS Letters, 1987
- cDNA and protein sequence of the NC1 domain of the α2‐chain of collagen IV and its comparison with α1(IV)FEBS Letters, 1986
- Proposed alignment of helical interruptions in the two subunits of the basement membrane (type IV) collagenFEBS Letters, 1986
- Structural model of the collagen-like region of C1q comprising the kink region and the fibre-like packing of the six triple helicesJournal of Molecular Biology, 1985
- Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectorsGene, 1985
- Structure of human‐basement‐membrane (type IV) collagenEuropean Journal of Biochemistry, 1984
- Localization of flexible sites in thread-like molecules from electron micrographsJournal of Molecular Biology, 1984
- Conformational implications of amino acid sequence regularities in collagenFEBS Letters, 1975
- The thermal transition of a non-hydroxylated form of collagen. Evidence for a role for hydroxyproline in stabilizing the triple-helix of collagenBiochemical and Biophysical Research Communications, 1973