Interactions of KB‐Cell Glycoproteins with an Adenovirus Capsid Protein

Abstract

Glycoprotein material extracted from human [oral carcinoma] KB cells with a flurocarbon, trichlorotrifluoroethane (Arklone P), into a water-soluble fraction binds to fiber, a structural protein of the adenovirus type-5 capsid. The fiber-binding glycoprotein(s) were purified by ion-exchange chromatography on DEAE-Sephadex and affinity chromatography on a fiber-Sepharose support. The purification procedure also includes a trypsinization step which eliminates the bulk of contaminating KB cell proteins present in the aqueous fraction without appreciably affecting the activity of the fiber-binding glycoproteins(s). Some comparison is made of the membrane-bound receptors for adenovirus and the water-soluble fiber-binding glycoprotein(s).