Amino‐terminal sequence of a Saccharomyces cerevisiae nuclear protein, NHP6, shows significant identity to bovine HMG1
- 26 September 1988
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 238 (1), 175-179
- https://doi.org/10.1016/0014-5793(88)80251-5
Abstract
Several nonhistone chromatin proteins (NHPs) have been isolated from Saccharomyces cerevisiae nuclei. They have molecular masses and amino acid compositions typical of the high mobility group (HMG) proteins from higher eukaryotic cells. Polyclonal antisera raised against two of the NHPs have been used in immunoblots of proteins from subcellular fractions of yeast to show that the NHPs are indeed nuclear. In addition, the amino-terminal amino acid sequences of several of the NHPs were determined. Importantly, the amino-terminal sequence of one of the proteins, NHP6, has significant (60%) identity with a stretch of amino acids in calf thymus HMG1.Keywords
This publication has 20 references indexed in Scilit:
- The distribution of nuclear proteins and transcriptionally-active sequences in rat liver chromatin fractionsExperimental Cell Research, 1986
- Isolation and initial characterization of residual nuclear structures from yeastExperimental Cell Research, 1984
- Improved technique utilizing nonfat dry milk for analysis of proteins and nucleic acids transferred to nitrocelluloseGene Analysis Techniques, 1984
- High mobility group proteins: abundance, turnover and relationship to transcriptionally active chromatinBiochemistry, 1983
- The organization of oligonucleosomes in yeastNucleic Acids Research, 1983
- Histone H2A subtypes associate interchangeably in vivo with histone H2B subtypes.Proceedings of the National Academy of Sciences, 1982
- Species and Tissue SpecificityPublished by Elsevier ,1982
- Isolation and AnalysisPublished by Elsevier ,1982
- High mobility group proteins of Saccharomyces cerevisiaeBiochemistry, 1980
- High mobility group proteins in yeastCarlsberg Research Communications, 1978