Ascorbate Biosynthesis in Arabidopsis Cell Suspension Culture
Open Access
- 1 October 1999
- journal article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 121 (2), 535-544
- https://doi.org/10.1104/pp.121.2.535
Abstract
The biosynthesis ofl-ascorbic acid (l-AA) in an Arabidopsis (L.) Heynh. cell suspension culture was studied by quantifying the effects of incubation with a range of potential biosynthetic precursors, analogs, and inhibitors on the intracellular levels of reduced and oxidized forms of l-AA. Our results support the recently published biosynthetic pathway of l-AA froml-galactose (G.L. Wheeler, M.A. Jones, N. Smirnoff [1998] Nature 393: 365–369), but suggest that Arabidopsis cell suspension culture simultaneously contains two other routes leading tol-AA. The possible physiological significance of these alternate routes is discussed.Keywords
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