Quantitative Chromatographic Analysis of the Phospholipids of Abnormal Human Red Blood Cells.

Abstract

The red blood cell phospholipids were analyzed in patients with hereditary spherocytosis, pernicious anemia, sprue, intermediate thalassemia, sickle cell anemia, and polycythemia vera using chromatography on silicic acid columns. The total lipid phosphorus concentration was increased in the patients with intermediate thalassemia and sickle cell anemia and may have been slightly decreased in the patients with pernicious anemia and sprue. No consistent difference from normal in distribution of the individual phospholipid fractions was found. Variations in the absolute concentrations of the individual phospholipid fractions reflected differences in the total red blood cell lipid phosphorus concentrations.