MONOCLONAL-ANTIBODIES TO CARCINOEMBRYONIC ANTIGEN - IONIC-STRENGTH AS A FACTOR IN THE SELECTION OF ANTIBODIES FOR IMMUNOSCINTIGRAPHY

Abstract

As part of an ongoing effort to improve the technique of immunoscintigraphy for the detection of human carcinomas with radiolabeled monoclonal antibodies (MAB) to carcinoembryonic antigen (CEA), a series of MAB to CEA were developed and the effects of low- and physiological molarity buffers on their CEA binding and affinity, their cross-reactivity with granulocyte glycoprotein(s) were studied. These in vitro results in different buffer systems were then correlated with the use of these MAB to CEA in the detection of human colon carcinoma grafts in nude mice. The binding of CEA by some MAB is influenced by ionic strength and this may be an important factor in their successful use for the immunolocalization of carcinomas in vivo.