Two separate tyrosine protein kinases in human platelets
Open Access
- 6 May 1985
- journal article
- Published by Wiley in FEBS Letters
- Vol. 184 (1), 56-59
- https://doi.org/10.1016/0014-5793(85)80652-9
Abstract
Tyrosine protein kinase activities were detected in the cytosolic fraction (PC‐TPK) and the particulate fraction (PM‐TPK) in human platelets using the synthetic peptide, E11G1, (Glu‐Asp‐Ala‐Glu‐Tyr‐Ala‐Ala‐Arg‐Arg‐Arg‐Gly) as a substrate. PC‐TPK and PM‐TPK were different in substrate specificities, divalent cation requirements and apparent M r values. These results strongly suggest that in platelets there exist at least two separate tyrosine protein kinases; one is present in cytosol and the other might be associated with membranes.Keywords
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