High-Performance Liquid Chromatography of the Tetradecapeptide Somatostatin

Abstract

Optimum reversed-phase systems for qualitative and quantitative determinations of somatostatin are evaluated. Temperature, pH, buffer concentration, type and concentration of organic modifier and the presence of ion-pairing agents more or less influence the retention and the separating efficiency. The retention behavior of some analogs is described to illustrate the selectivity of the system. Down to 10-20 ng of somatostatin can be determined, even in the presence of a large excess of albumin, by using 210 nm as the detection wavelength.