An apparatus for the determination of molecular weights by light scattering

Abstract

The intensity of the light scattered by solutions of large molecules is frequently used as a measure of their weight-average molecular weight. The principles underlying the design of an instrument, and the detailed method of construction, are given. The cell for holding the liquid under examination differs entirely in design from those currently employed elsewhere. The measured signal from a solution is virtually free from extraneous light. This is due to the internal light absorption characteristics of the cell and to the careful positioning of the centre of observation. The principles of this cell design called for a number of mechanical and optical features unlike those encountered in conventional light-scattering apparatus.