A stable and simple method of "faglupagas fixation" for catecholamines for routine examination.

Abstract

Various techniques of fluorescence histochemistry have some methodological difficulties mainly due to the instability of amine fluorophores. This study aims to develop a stable and relatively simple histofluorescence method which can be applied to the routine examination for diagnostic histopathologists. Dissected [rat and human] tissue pieces were fixed by immersing in a FAGLUPAGAS [paraformaldehyde, glutaraldehyde, picric acid, glyoxylic acid and sucrose] solution overnight. Cryostat sections of fixed materials were washed in a FAGLUGAS [FAGLUPAGAS without picric acid] solution, mounted on glass slides, dried by warm air and finally cover-slipped in Entellan. Amine fluorescences in fixed tissues were stable for several months when the specimens, either tissue blocks or cryostat sections, were kept in the FAGLUPAGAS.