Cholera Toxin-Peroxidase: Changes in Surface Labeling of Glioblastoma Cells with Increased Time in Tissue Culture

Abstract
Cholera toxin coupled to peroxidase yielded a highly specific ultrastructural marker of plasma membrane monosialogangliosides. Studies with cultures of brain and brain tumors suggested that long-term culture of tissue in monolayers results in eventual loss of surface monosialogangliosides.