Ceramide induces apoptosis via CPP32 activation
- 21 October 1996
- journal article
- Published by Wiley in FEBS Letters
- Vol. 395 (2-3), 267-271
- https://doi.org/10.1016/0014-5793(96)01050-2
Abstract
Although both ceramide and interleukin-1β converting enzyme (ICE) family proteases are key molecules during apoptosis, their relationship remains to be elucidated. We report here that cell-permeable ceramide induced cleavage and activation of CPP32, a Ced-3/ICE-like protease, but not ICE. Ceramide-induced apoptosis of Jurkat cells was blocked by the CPP32-specific tetrapeptide inhibitor DEVD-CHO, but not by the ICE inhibitor YVAD-CHO. Furthermore, variant Jurkat cells with defective CPP32 activation were resistant to both antiFas- and ceramide-induced apoptosis. These results indicate that CPP32 activation is required for ceramide-induced apoptosis, and suggest sphingomyelin-ceramide pathway functions upstream of CPP32.Keywords
This publication has 34 references indexed in Scilit:
- Sequential activation of ICE-like and CPP32-like proteases during Fas-mediated apoptosisNature, 1996
- ICE Family Proteases: Mediators of All Apoptotic Cell Death?Immunity, 1996
- CPP32/Apopain Is a Key Interleukin 1β Converting Enzyme-like Protease Involved in Fas-mediated ApoptosisJournal of Biological Chemistry, 1996
- FAS-induced apoptosis is mediated via a ceramide-initiated RAS signaling pathwayImmunity, 1995
- Altered Cytokine Export and Apoptosis in Mice Deficient in Interleukin-1β Converting EnzymeScience, 1995
- Programmed Cell Death Induced by CeramideScience, 1993
- A New Sulfonated Tetrazolium Salt That Produces a Highly Water-Soluble Formazan Dye.CHEMICAL & PHARMACEUTICAL BULLETIN, 1993
- Tumor necrosis factor α stimulates sphingomyelinase through the 55 kDa receptor in HL‐60 cellsFEBS Letters, 1992
- A novel heterodimeric cysteine protease is required for interleukin-1βprocessing in monocytesNature, 1992
- Tumor Necrosis Factor-α Activates the Sphingomyelin Signal Transduction Pathway in a Cell-Free SystemScience, 1992