Localization of phospholamban in smooth muscle using immunogold electron microscopy.
Open Access
- 1 August 1988
- journal article
- research article
- Published by Rockefeller University Press in The Journal of cell biology
- Vol. 107 (2), 555-562
- https://doi.org/10.1083/jcb.107.2.555
Abstract
Phospholamban, the putative regulator of the Ca2+-ATPase in cardiac sarcoplasmic reticulum, was immunolocalized in canine visceral and vascular smooth muscle. Gently disrupted tissues were labeled with an affinity-purified phospholamban polyclonal antibody and indirect immunogold, using preembedding techniques. The sarcoplasmic reticulum of smooth muscle cells was specifically labeled with patches of immunogold distributed in a nonuniform fashon, while the sarcolemma did not appear to contain any phosholamban. The outer nuclear envelopes were also observed to be heavily labeled with the affinity-purified phospholamban polyclonal antibody. These findings suggest that phospholamban may play a role in the regulation of cytoplasmic and intranuclear calcium levels in smooth muscle cells.This publication has 31 references indexed in Scilit:
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