Phosphatidylinositol Cycle Metabolites in Samanea saman Pulvini
Open Access
- 1 March 1987
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 83 (3), 640-644
- https://doi.org/10.1104/pp.83.3.640
Abstract
Ten monoclonal antibodies (McAbs) have been produced against the cationic peroxidase from peanut suspension cell culture. Eight of these antibodies were found to be of the immunoglobulin (Ig)G1 subclass and two were of IgA subclass. A combination of competitive enzyme-linked immunosorbent assay, Western blotting analysis, and direct antigen-binding assay revealed that the antibodies are directed against four different epitopes on the cationic peroxidase and the McAbs can be subdivided into four groups. Only group A inhibits peroxidase activity. Group B and D bind equally well to the native and the denatured form of cationic peroxidase, whereas the remaining McAbs react with more or less reduced affinity to the denatured antigen. Group C probably recognizes a conformation-dependent epitope. All the McAbs cross react weakly with the anionic peanut peroxidase, suggesting a structural nonidentity as well as some similarity between these two peroxidase isozymes. Cross reactivities of these McAbs with peroxidases of various plant species were also demonstrated.This publication has 19 references indexed in Scilit:
- Polyphosphoinositides are present in plant tissue culture cellsBiochemical and Biophysical Research Communications, 1985
- Release of Ca2+ from plant hypocotyl microsomes by inositol-1,4,5-trisphosphateBiochemical and Biophysical Research Communications, 1985
- Biochemical Modeling of an Autonomously Oscillatory Circadian Clock in EuglenaScience, 1985
- Evidence of regulation of calcium uptake by phytochrome in maize protoplastsBiochemical and Biophysical Research Communications, 1985
- Inositol trisphosphate, a novel second messenger in cellular signal transductionNature, 1984
- Photoreceptor excitation and adaptation by inositol 1,4,5-trisphosphateNature, 1984
- The polyphosphoinositide phosphodiesterase of erythrocyte membranesBiochemical Journal, 1981
- Pituitary hormones influence polyphosphoinositide metabolism in rat brainFEBS Letters, 1979
- Circadian Rhythmicity in Excised Samanea PulviniPlant Physiology, 1976
- RAPIDLY‐LABELLED, ACIDIC PHOSPHOLIPIDS OF THE GOLDFISH BRAIN1Journal of Neurochemistry, 1970