1. Ether extracts of nodules of bean and pea were more active in effecting negative curvatures of coleoptiles in the Avena test than those of denodulated roots. These in turn were more active than those of roots grown in sterilized quartz sand. 2. The same relations, but less marked, were found for nodules and roots of soybean. 3. Negative bending activity of extracts of nodules of bean and pea and of sterile pea roots was increased by agar dilution. This may indicate a fraction in each extract which changes to auxin by hydrolysis, and that the activity noted is a measure of a positive auxin balance between fractions antagonistic in their effect on cell elongation in the coleoptile. The dilution effect was most pronounced in extracts of nodules of bean and pea. 4. Extracts of nodules and of roots of bean contained a fraction which behaved like auxin a in acid hydrolysis. The nodule extract contained more of it than the root extract. 5. Extracts of nodules and of roots of bean contained a fraction which was auxinic after alkali hydrolysis. The nodule extract contained more of it than the root extract. This fraction may be composed of auxin present before hydrolysis which behaves like indoleacetic acid in alkali hydrolysis, of auxin derived from a precursor by hydrolysis, or of both. 6. It is inferred that the nodules of bean, soybean, and pea have greater and different auxone contents than the roots which bear them, and that these in turn have greater and different auxone contents than the roots when grown in sterilized substrates.