A DNA-RNA complex as an intermediate of in vitro genetic transcription.
- 1 December 1965
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 54 (6), 1736-1743
- https://doi.org/10.1073/pnas.54.6.1736
Abstract
In vitro transcription of RNA message on K174 replicative circular DNA was studied. Results are summarized as follows: During the transcription, a double-strand DNA and RNA hybrid could be detected. An RNase-resistant structure was combining the template DNA and synthesized RNA. This RNase-resistant structure moved along the DNA and existed at the region where polyrlbonucleotide chains grew. A portion of RNA synthesized was polycistronic as judged by its sedimentation constant. A model for the mechanism of genetic transcription in vitro was proposed.This publication has 30 references indexed in Scilit:
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