Subunit Structure ofMicrococcus luteusCatalase. Dissociation of M.luteusCatalase Induced by Dodecylsulfate, Citraconic and 2,3-Dimethylmaleic Anhydrides and Urea
- 1 January 1978
- journal article
- research article
- Published by Walter de Gruyter GmbH in Hoppe-Seyler´s Zeitschrift Für Physiologische Chemie
- Vol. 359 (2), 857-862
- https://doi.org/10.1515/bchm2.1978.359.2.857
Abstract
M. luteus catalase dissociates upon treatment with urea, dodecylsulfate and anhydrides into monomers, the MW of which appears to be 1/4 of that of the native enzyme. The urea-induced dissociation depends upon the incubation time, the urea concentration and the pH of the incubation mixture. Reassociation of the subunits proved to be unsuccessful. Native M. luteus catalase only contains 30% .alpha.-helix. When fully dissociated in presence of urea, it still retains 15% .alpha.-helix. Catalase from M. luteus lacked cysteine residues.This publication has 13 references indexed in Scilit:
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