Production of sakacin P by Lactobacillus sakei in a completely defined medium
Open Access
- 1 March 2000
- journal article
- research article
- Published by Wiley in Journal of Applied Microbiology
- Vol. 88 (3), 536-545
- https://doi.org/10.1046/j.1365-2672.2000.00994.x
Abstract
In order to investigate factors influencing the production of the bacteriocin, sakacin P, Lactobacillus sakei CCUG 42687 was grown in a completely defined medium (DML‐B) with 33 components. Although the maximum sakacin P concentration obtained was higher on a complex medium due to higher cell mass, the production per cell mass was higher in DML‐B. Sakacin P was produced at 4–30 °C, with the highest specific production at low temperatures. More sakacin P was produced at uncontrolled pH compared with cultivation at pH 6·3. Tween‐80 had a positive effect on sakacin P production, while addition of sodium chloride and trace metals had negative effects. The decrease in sakacin P concentration during the late growth and stationary phases was shown to be cell‐independent and promoted at high temperature and pH. Some differences in production levels of sakacin P were found among six strains of Lactobacillus sakei tested.Keywords
This publication has 25 references indexed in Scilit:
- A system for heterologous expression of bacteriocins in Lactobacillus sakeFEMS Microbiology Letters, 1998
- Pheromone‐induced production of antimicrobial peptides in LactobacillusMolecular Microbiology, 1997
- Analysis of the sakacin P gene cluster from Lactobacillus sake Lb674 and its expression in sakacin-negative Lb. sake strainsMicrobiology, 1996
- Induction of bacteriocin production in Lactobacillus sake by a secreted peptideJournal of Bacteriology, 1996
- Primary metabolite kinetics of bacteriocin biosynthesis by Lactobacillus amylovorus and evidence for stimulation of bacteriocin production under unfavourable growth conditionsMicrobiology, 1996
- Tween 80 effect on bacteriocin synthesis by Lactococcus lactis subsp. cremoris J46Letters in Applied Microbiology, 1996
- Nutritional factors affecting nisin production by Lactococcus lactis subsp. actis NIZO 22186 in a synthetic mediumJournal of Applied Bacteriology, 1995
- Purification and cloning of sakacin 674, a bacteriocin from Lactobacillus sake Lb674FEMS Microbiology Letters, 1994
- The influence of pH and growth rate on production of the bacteriocin, bavaricin MN, in batch and continuous fermentationsJournal of Applied Bacteriology, 1993
- Influence of the phosphorus and nitrogen source on nisin production in Lactococcus lactis subsp. lactis batch fermentations using a complex mediumApplied Microbiology and Biotechnology, 1993