Immunofluorescent detection of differentiation alloantigens (CA1) in the chicken

Abstract

Lymphoid cell surface alloantigens were detected by a triple‐layer immunofluorescence technique. An antiserum raised against B locus‐incompatible lymphoid cells also reacted with previously undefined differentiation antigens (CA1) which segregated between B locus homozygous (B¹⁴/B¹⁴) chickens of the same strain as the antiserum donor. CAl‐positive chickens reacted with the antiserum by agglutination of red cells and staining of all peripheral lymphocytes and thymocytes but not bursal cells. Cross‐absorption experiments have demonstrated that antigens expressed by red cells, T cells (both thymic and peripheral) and B cells (peripheral only) respectively, were of distinct specificity.