The relationship between molecular structure and transformation efficiency of some S. aureus plasmids isolated from B. subtilis
- 1 January 1978
- journal article
- research article
- Published by Springer Science and Business Media LLC in Molecular Genetics and Genomics
- Vol. 166 (3), 259-267
- https://doi.org/10.1007/bf00267617
Abstract
Summary DNA preparations of the chloramphenicol resistance determining S. aureas plasmids pC194, pC223, and PUB112 can be fractionated by gel electrophoresis into various bands. Electromicroscopic investigations of these various molecular species obtained with pC194 indicated that, depending on the preparations, 70 to 80% of the molecules were monomers, while the rest consisted of various classes of concatemeric and/or interlocked multimers. Measurements of the specific transforming activity of the various molecular classes indicated that the monomers had less than one thousandth the activity of the multimeric plasmid DNA. pC194 DNa of high specific transforming activity could also be obtained by ligation of HindIII generated monomers into concatemeric DNA.Keywords
This publication has 28 references indexed in Scilit:
- On the dose response in B. subtilis transfectionMolecular Genetics and Genomics, 1977
- A freeze-squeeze method for recovering long DNA from agarose gelsAnalytical Biochemistry, 1975
- A catenated intermediate in plasmid replicationBiochemical and Biophysical Research Communications, 1973
- A catenated DNA molecule as an intermediate in the replication of the resistance transfer factor R6K in Escherichia coliBiochemical and Biophysical Research Communications, 1973
- Transfection in B. subtilisPublished by Springer Science and Business Media LLC ,1973
- Fate of transforming DNA following uptake by competent Bacillus subtilis: III. Formation and properties of products isolated from transformed cells which are derived entirely from donor DNAJournal of Molecular Biology, 1972
- Studies on the structure and formation of polyoma DNA replicative intermediatesJournal of Molecular Biology, 1971
- Isolation of circular DNA by zonal centrifugation: Separation of normal length, double length and catenated M13 replicative form DNA and of host specific “episomal” DNABiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1969
- Catenated Circular DNA Molecules in HeLa Cell MitochondriaNature, 1967
- Physical properties of the DNA of bacteriophage SP50Molecular Genetics and Genomics, 1967