Comparison of the Roughton-Scholander Syringe and Polarographic Methods for Measuring Tension of Oxygen in Plasma and Whole Blood

Abstract

A method for measuring O2 tensions within the physiologic range of 0 to 100 mm Hg was developed by the use of the polarograph. A description of a container in which to centrifuge blood without the loss of O2 tension is included in the manuscript. O2 consumption of normal whole blood at a tension of 150 mm Hg is sufficient to decrease the level of O2 tension at a rate of 1 mm Hg/minute. The efficiency of the polarographic and Roughton-Scholander syringe methods of measuring O2 tension in whole blood was compared and found to be the same__R. H. Wilson.