A cyclotron target system for the production of anhydrous 18F- was developed and used for the synthesis of carrier-free [18F]-3-deoxy-3-fluoro-D-glucose (3-FDG). The synthesis is sufficiently rapid and efficient to allow production of usable amounts of 3-FDG with a 6-MeV cyclotron. Preliminary animal [rhesus monkey, Macaca mulatta] studies show that 3-FDG is in fact a glucose analog.