125I‐d(CH2)5[Tyr(Me)2,Tyr(NH2)9]AVP: Iodination and binding characteristics of a vasopressin receptor ligand
- 14 March 1988
- journal article
- Published by Wiley in FEBS Letters
- Vol. 229 (2), 251-255
- https://doi.org/10.1016/0014-5793(88)81135-9
Abstract
A radioiodinated vasopressin antagonist, d(CH2)5[Tyr(Me)2,Tyr(NH2)9]AVP has been prepared. Iodination was carried out at the phenyl moiety of the tyrosylamide residue at position 9, followed by HPLC purification. Non-radiolabelled monoiodinated antagonist was used as a reference for identification. 125I-d(CH2)5[Tyr(Me)2,Tyr(NH2)9]AVP binding appeared to take place with a dissociation constant of 0.28±0.09 nM (K d±SD) to V1 vasopressin receptors on rat liver membranes.Keywords
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