Enhanced chemiluminescence in the peroxidase‐luminol‐H2O2 system: Anomalous reactivity of enhancer phenols with enzyme intermediates
- 1 January 1989
- journal article
- research article
- Published by Wiley in Journal of Bioluminescence and Chemiluminescence
- Vol. 3 (1), 21-25
- https://doi.org/10.1002/bio.1170030105
Abstract
Phenols which markedly enhance chemiluminescence in the horseradish peroxidase catalysed oxidation of luminol by hydrogen peroxide show anomalously high reactivity (by factors of ∼102 compared with published Hammett correlations) in the reduction of the enzyme intermediates, Compound I and Compound II. The results support the hypothesis that efficient production of phenoxy radicals from phenols is a necessary criterion for chemiluminescence enhancer action.Keywords
This publication has 10 references indexed in Scilit:
- Hammett ϱσ correlation for reactions of horseradish peroxidase compound II with phenolsArchives of Biochemistry and Biophysics, 1986
- [29] Enhanced chemiluminescent reactions catalyzed by horseradish peroxidaseMethods in Enzymology, 1986
- Phenols as enhancers of the chemiluminescent horseradish peroxidase-luminol-hydrogen peroxide reaction: application in luminescence-monitored enzyme immunoassays.Clinical Chemistry, 1985
- Oxidation of p-cresol by horseradish peroxidase compound I.Journal of Biological Chemistry, 1976
- Substituent Effect on the Oxidation of Phenols and Aromatic Amines by Horseradish Peroxidase Compound IEuropean Journal of Biochemistry, 1976
- An Investigation of the Mechanism of the Luminescent Peroxidation of Luminol by Stopped Flow TechniquesJournal of Biological Chemistry, 1968
- 69. The ionisation constants of some substituted anilines and phenols: a test of the Hammett relationJournal of the Chemical Society, 1961
- An Extended Table of Hammett Substitutent Constants Based on the Ionization of Substituted Benzoic AcidsThe Journal of Organic Chemistry, 1958
- A Reëxamination of the Hammett Equation.Chemical Reviews, 1953