TRANSFORMATION INDUCED BY PR 8 INFLUENZA VIRUS IN PRIMARY CULTURES OF MOUSE KIDNEY AND BRONCHUS, AND PRODUCTION OF MALIGNANT KIDNEY TUMORS IN MICE BY SUBCULTURES

Abstract
Cultures from kidney and bronchus of Snell''s mice when infected with PR 8 influenza virus, showed essentially the same sequence of cytological and cytochemical alterations as those observed in bronchi and lungs of CF1 mice after in vivo infection with PR 8 influenza virus. In addition, there occurred in the infected tissue cultures giant bizarrely shaped cells resembling closely those described by Shein and Enders for cells from human kidney cultures infected with SV 40 virus. Intraperitoneal and intrathoracic injection of newbron mice with subcultures derived from PR 8 infected explants of kidney and bronchus resulted in production of malignant kidney tumors.