Transcription of the αA‐globin gene of the duck
- 1 July 1989
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 183 (1), 145-153
- https://doi.org/10.1111/j.1432-1033.1989.tb14906.x
Abstract
A homologous in vitro transcription system was developed in which the .alpha.A-globin gene of the duck was faithfully transcribed. Whole-cell extracts from duck erythrocytes were separated into fractions A, B, C and D by consecutive elution from phosphocellulose columns and were individually reconstituted in run-off transcription assays. Fractions A, C and D required to achieve faithful initiation on the .alpha.A-globin gene. The latter fractions were mutually interchangeable with comparable fractions from HeLa cells. A fourth fraction, B, was not required but enhanced basal transcription when reconstituted with fractions A, C and D or a very low amount of HeLa whole-cell extract which by itself did not yield a detectable signal. Fraction B from duck erythrocytes was further purified by chromatography on DEAE-Sephadex and was shown to contain two trans-acting factors. One of these differentially acts on the .alpha.A-globin gene of the duck. The other component from duck erythrocytes surprisingly resembles the upstream stimulatory factor, previously isolated from HeLa cells. This latter protein binds to the trans-activates the adenovirus 2 major late promoter, but is not involved in the transcription of the .alpha.A-globin gene.This publication has 45 references indexed in Scilit:
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