Energy metabolism of skeletal muscle biopsies stimulated anaerobically without load in vitro
- 1 June 1986
- journal article
- research article
- Published by American Physiological Society in American Journal of Physiology-Cell Physiology
- Vol. 250 (6), C813-C820
- https://doi.org/10.1152/ajpcell.1986.250.6.c813
Abstract
This study was made to test the validity of a simple biopsy technique for assessing the metabolic capacity of skeletal muscle. The biopsy is stimulated under mineral oil without attachment, i.e., without load or tension, then freeze-clamped and assayed for ATP, phosphocreatine, glucose 6-phosphate, and lactate. The mineral oil creates a closed anaerobic system. Background studies demonstrated in the absence of a load, metabolic changes with stimulation were little affected by cutting the fibers to obtain the biopsy; and high-energy phosphate (approximately P) consumption during a brief tetanus was not much lower than that for an isometric tetanus. Individual fast-twitch oxidative-glycolytic (IIA) and fast-twitch glycolytic (IIB) fibers obtained from the freeze-clamped biopsy showed distinct differences in approximately P consumption and metabolic changes. The results indicate that this technique could be useful for studies of normal and pathological human muscle.This publication has 12 references indexed in Scilit:
- Energetics of isometric contractions as a function of muscle temperatureAmerican Journal of Physiology-Cell Physiology, 1983
- Contractile properties of bundles of fiber segments from skeletal musclesAmerican Journal of Physiology-Cell Physiology, 1982
- Metabolite changes in individual rat muscle fibers during stimulationAmerican Journal of Physiology-Cell Physiology, 1982
- Chemical energetics of slow- and fast-twitch muscles of the mouse.The Journal of general physiology, 1982
- Enzyme levels in individual rat muscle fibersAmerican Journal of Physiology-Cell Physiology, 1980
- Reversal of phosphorylase activation in muscle despite continued contractile activityAmerican Journal of Physiology-Regulatory, Integrative and Comparative Physiology, 1979
- DIVERSITY OF METABOLIC PATTERNS IN HUMAN BRAIN TUMORS—I. HIGH ENERGY PHOSPHATE COMPOUNDS AND BASIC COMPOSITION1Journal of Neurochemistry, 1977
- A Study of the Contractility, Biochemistry and Morphology of an Isolated Preparation of Human Skeletal MuscleClinical Science, 1977
- Slow and fast twitch fibers in human skeletal muscleAmerican Journal of Physiology-Legacy Content, 1968
- The Relationships between Substrates and Enzymes of Glycolysis in BrainJournal of Biological Chemistry, 1964