Brief Communication: Growth of Human Normal and Neoplastic Mammary Tissues in the Cleared Mammary Fat Pad of the Nude Mouse2

Abstract

Dysplastic and malignant human breast tissues were grown successfully in the cleared mammary fat pads (CFP) of nude mice. The mammary fat pads were cleared while the mice were in a germfree isolator. Prepared mice were removed from the germfree environment to facilitate transplantation of the human mammary tissue into their CFP and subsequently were maintained in sterile laminar flow racks.