Spread of Integron-Associated VIM-Type Metallo-β-Lactamase Genes among Imipenem-Nonsusceptible Pseudomonas aeruginosa Strains in Greek Hospitals
- 1 February 2003
- journal article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 41 (2), 822-5
- https://doi.org/10.1128/jcm.41.2.822-825.2003
Abstract
Fifty-eight imipenem-nonsusceptible (MIC ≥ 8 μg/ml) Pseudomonas aeruginosa strains isolated during May 2001 in 15 Greek hospitals were studied. Thirty-six isolates derived from nine hospitals carried VIM-type metallo-β-lactamase genes, as found by PCR. In 34 isolates, bla VIM was associated with class 1 integrons of various sizes. DNA sequencing indicated the presence of bla VIM-2 gene cassettes in a variety of integron structures. Random amplified polymorphic DNA typing suggested diversity of the bla VIM -positive strains. Synergy between 2-mercaptoacetic acid and imipenem indicated carbapenemase activity in 26 bla VIM -positive strains.Keywords
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