FRACTIONATION OF PHYTOHEMAGGLUTININ, I. PURIFICATION OF THE RNA AND DNA SYNTHESIS-STIMULATING SUBSTANCES AND EVIDENCE THAT THEY ARE NOT PROTEINS
- 1 September 1969
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 64 (1), 283-289
- https://doi.org/10.1073/pnas.64.1.283
Abstract
We have separated the RNA synthesis-stimulating activity and the DNA synthesis-stimulating activity of extracts of Phaseolus vulgaris (phytohemagglutinin) from the erythroagglutinins, leukoagglutinins, and cytotoxic factors. We have shown that the substances stimulating the synthesis of nucleic acids are probably not proteins, since they are not significantly affected by rigorous deproteinization or treatment by trypsin and pronase. Likewise, they do not appear to be nucleic acids, since they are not precipitated by perchloric acid and resist RNase, DNase, and phosphodiesterases. They are probably not carbohydrates, since 98 per cent of the carbohydrate can be removed from a preparation with only a moderate loss in activity. The RNA and DNA synthesis-stimulating activities are probably associated with separate molecules, since they are destroyed by periodate at different rates.Keywords
This publication has 10 references indexed in Scilit:
- The dissociation of phytohemagglutinin of Phaseolus vulgaris by 8.0 M urea and the separation of the mitogenic from the erythroagglutinating activityBiochemical and Biophysical Research Communications, 1969
- Early effects of phytohaemagglutinin on glucose metabolism of normal human lymphocytesBiochemical Journal, 1968
- SEPARATION OF VIABLE LYMPHOCYTES FROM HUMAN BLOODThe Lancet, 1964
- STUDIES ON THE PHYTOHEMAGGLUTININ OF PHASEOLUS VULGARIS AND ITS MITOGENICITY*Annals of the New York Academy of Sciences, 1964
- Isolation of Immunologically Competent Lymphocytes from Sensitized Mouse Spleens.Experimental Biology and Medicine, 1964
- Phytohæmagglutinin: Separation of Hæmagglutinating and Mitogenic PrinciplesNature, 1963
- Application of a Microtechnique to Viral Serological InvestigationsThe Journal of Immunology, 1962
- New Color Reactions for Determination of Sugars in PolysaccharidesPublished by Wiley ,1955