Quantitation of Branched Chain α-Keto Acids in Sheep Plasma Using Reversed-Phase High Performance Liquid Chromatography and Quinoxalinol Derivatization

Abstract

The concentration of branched chain α-keto acids in sheep plasma was determined by reversed-phase high performance liquid chromatography using precolumn quinoxalinol formation and ion exchange chromatography for sample preparation. The clear resolution, high degree of precision and accuracy, and relatively simple sample cleanup and derivatization procedures render this technique suitable for the routine analysis of physiological fluids.