ATP Synthesis Driven by a Valinomycine Induced K+ Diffusion Potential in Liposomes Bearing Chloroplast ATP Synthase

Abstract

Partially purified chloroplast ATP synthase was reconstituted into asolectin liposomes. A valinomycine induced potassium diffusion potential from outside to inside the vesicles promoted a measurable ATP synthesis. If valinomycine was replaced by nigericine, practically no ATP was formed