Alternative mechanized method for analysis for inorganic phosphate without reduction.

Abstract

The mechanized measurement of inorganic phosphate in plasma can be achieved without reduction by only minor alterations to an existing continuous-flow reduction procedure. The method is based on the enhancement of the extinction of the phosphomolybdate complex by the detergent Triton X-100. Results obtained agree well with those obtained by the standard reduction (Fiske-SubbaRow) procedure. The correlation coefficient between the two methods was 0.996 and statistical analysis of the data confirmed very good agreement between the two sets of values.