MICROBIOLOGICAL ASSAY FOR THIAMIN USING LACTOBACILLUS VIRIDESCENS
- 1 December 1957
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 74 (6), 818-821
- https://doi.org/10.1128/jb.74.6.818-821.1957
Abstract
This method is convenient, rapid, relatively specific, and less subject to interfering substances as compared to previously described methods for determination of this vitamin. Cocarboxylase has about 60% of the activity of equimolar quantities of thiamine. The pyrimidine and thiazole moieties of thiamine are not utilized, either individually or together, and the results are not affected by reasonable levels of pentoses, fructose, maltose, calcium, thioglycollate, or acetate.Keywords
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