Abstract
A method is described for the direct bioassay of biotin in seawater samples. The assay organism is an unidentified marine bacterium that shows a growth response, in enriched seawater medium, proportional to dissolved biotin concentration in the range 3–12 μμg/ml. The growth was measured turbidimetrically with a spectrophotometer. The effective assay range was extended successfully to higher biotin levels by making appropriate dilutions in synthetic seawater.

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