Reconstructing 3-D light-microscopic images by digital image processing
- 15 January 1985
- journal article
- Published by Optica Publishing Group in Applied Optics
- Vol. 24 (2), 194-200
- https://doi.org/10.1364/ao.24.000194
Abstract
A reconstruction procedure based on linear system theory has been developed for 3-D light-microscopic images. Inverse filtering with the 3-D optical transfer function was used for image reconstruction. The procedure allows a significant improvement in spatial resolution in the image planes perpendicular to the optical axis.This publication has 5 references indexed in Scilit:
- Characteristic folding pattern of polytene chromosomes in Drosophila salivary gland nucleiNature, 1984
- Three-dimensional architecture of a polytene nucleusNature, 1983
- Properties of a Defocused Optical System*Journal of the Optical Society of America, 1969
- An algorithm for the machine calculation of complex Fourier seriesMathematics of Computation, 1965
- The frequency response of a defocused optical systemProceedings of the Royal Society of London. Series A. Mathematical and Physical Sciences, 1955