Rabbit erythrocyte band 3: a receptor for staphylococcal alpha toxin
Open Access
- 1 April 1980
- journal article
- research article
- Published by Canadian Science Publishing in Canadian Journal of Microbiology
- Vol. 26 (4), 524-531
- https://doi.org/10.1139/m80-088
Abstract
Enzymes known to specifically cleave the band 3 component of the rabbit erythrocyte membrane reduced both the hemolytic sensitivity to and the binding of the .alpha. toxin of Staphylococcus aureus. Lectins which bind to band 3 also inhibited the toxin. Lectins which do not bind to band 3 have no effect. Purified band 3, isolated by affinity chromatography on a concanavalin A column, was homogeneous by polyacrylamide gel electrophoresis, had a MW of 100,000, and inhibited the hemolytic activity of .alpha. toxin. Antibodies to the toxin-toxoid receptor were serologically indistinguishable from antiband 3.This publication has 16 references indexed in Scilit:
- The band 3 protein of the human red cell membrane: A reviewJournal of Supramolecular Structure, 1978
- Glycophorin and the concanavalin A receptor of human erythrocytes: their receptor function in lipid bilayers.Proceedings of the National Academy of Sciences, 1977
- Peptide heterogeneity in the band 3 protein of rabbit erythrocyte plasma membranesBiochemical and Biophysical Research Communications, 1976
- Studies on the binding of staphylococcal 125I-labeled α-toxin to rabbit erythrocytesBiochemistry, 1976
- A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye BindingAnalytical Biochemistry, 1976
- Proteolytic dissection of band 3, the predominant transmembrane polypeptide of the human erythrocyte membraneBiochemistry, 1976
- Isolation of membrane glycoproteins by affinity chromatography in the presence of detergentsBiochimica et Biophysica Acta (BBA) - Biomembranes, 1976
- Staphylococcal Alpha-Hemolysin: Detection on the Erythrocyte Membrane by ImmunofluorescenceScience, 1964
- The preparation and chemical characteristics of hemoglobin-free ghosts of human erythrocytesArchives of Biochemistry and Biophysics, 1963
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951