Histone H2A phosphorylation in animal cells: functional considerations
- 1 May 1982
- journal article
- research article
- Published by American Chemical Society (ACS) in Biochemistry
- Vol. 21 (10), 2412-2420
- https://doi.org/10.1021/bi00539a021
Abstract
The role of histone H2A phosphorylation in chromatin was studied by examining the distribution of the phosphorylated and unphosphorylated forms of this core histone within the nuclei of mouse and human cells. At any time, only about 15% of the H2A of whole chromatin is in the phosphorylated form, and its phosphate is rapidly turned over, even in quiescent cells that contain a functional nucleus. The phosphorylations and dephosphorylations are not specifically related to progress through the cell cycle, nor to DNA synthesis or repair, and they are not selectively nucleolar. Euchromatin is substantially enriched with phosphorylated H2A but is not the exclusive repository of it. Possible roles of this modification of H2A are considered.This publication has 24 references indexed in Scilit:
- Nuclear protein synthesis and phosphorylation in Friend erythroleukemia cells stimulated with DMSOExperimental Cell Research, 1978
- Methylated 5′-terminal sequences of vaccinia virus mRNA species made in Vivo at early and late times after infectionVirology, 1977
- Selective digestion of transcriptionally active ovalbumin genes from oviduct nuclei.Proceedings of the National Academy of Sciences, 1976
- Changes of chromatin structure induced by acid mucopolysaccharidesExperimental Cell Research, 1976
- Chromosomal Subunits in Active Genes Have an Altered ConformationScience, 1976
- Phosphorylation of distinct regions of f1 histone. Relationship to the cell cycle.Journal of Biological Chemistry, 1976
- A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye BindingAnalytical Biochemistry, 1976
- Selective synthesis and modification of nuclear proteins during maturation of avian erythroid cellsArchives of Biochemistry and Biophysics, 1976
- RNA metabolism in the HeLa cell nucleusJournal of Molecular Biology, 1966
- A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acidBiochemical Journal, 1956