A Controllable Carmine Technic for Plants with Small Chromosomes
- 1 January 1952
- journal article
- research article
- Published by Taylor & Francis in Stain Technology
- Vol. 27 (6), 333-336
- https://doi.org/10.3109/10520295209105098
Abstract
Anthers of small chromosome plants (Antirrhinum, Brassica, Capsicum etc.) were fixed 12 hours or longer at 0–3° C. in: ferric acetate in glacial acetic acid (sat. soln.), 1 part; absolute alcohol, 3 parts. They were transferred to: ferric acetate (sat. soln.) in 45% acetic acid, 3 parts; 45% acetic acid, 5 parts; 1% formalin (aq.), 2 parts, and allowed to remain 5–15 minutes at room temperature for mordanting. The amount of iron introduced into the specimens was controllable by the time in the mordanting fluid. After rinsing the specimen in 45% acetic acid and macerating in a drop of Belling's acetocarmine on a slide, a cover slip was applied followed by warming and pressing with blotting paper to flatten the pollen mother cells and expel excess stain. Preparations stored temporarily by sealing the edges of the cover slip with rubber solution were best made permanent by removing the cover slip after 1–2 days, dehydrating and mounting in euparal.Keywords
This publication has 3 references indexed in Scilit:
- Basic Fuchsin as a Nuclear Stain for FungiMycologia, 1948
- The Aceto-Carmine Method for Fruit MaterialStain Technology, 1940
- PERMANENT ACETO-CARMINE PREPARATIONSScience, 1935