Aminoacylation of Encephalomyocarditis Virus RNA

Abstract
Summary RNA extracted from purified encephalomyocarditis (EMC) virus (EMC-RNA) can be aminoacylated with synthetase preparations from Escherichia coli, beef and rabbit liver. The extent of aminoacylation is between 0·024 and 0·080 moles per mole EMC-RNA and occurs only with serine. Neither removal of possible low mol. wt. contaminants with 3 m-sodium acetate nor periodate oxidation of the virus RNA affects its aminoacylation capacity.