Immunological studies of ?-adrenergic receptors
- 1 January 1983
- journal article
- research article
- Published by Wiley in Journal of Cellular Biochemistry
- Vol. 21 (3), 187-193
- https://doi.org/10.1002/jcb.240210301
Abstract
Two types for antibodies have been raised against the β-adrenergic receptor: either by injection of highly purified receptor from turkey erythrocytes or by injection of anticatecholamine ligand antibodies, and induction of anti-idiotypic antibodies Our data illustrate the interactions of the β-adrenergic receptor with these polyclonal antibodies. Preliminary results with monoclonal antibodies are also described. The redistribution of β-receptors on intact cells is visualized by the use of fluorescent antibodies. Immunoprecipitation of radioiodinated receptor by the antireceptor antibodies yields a single major 60,000 MW component.Keywords
This publication has 19 references indexed in Scilit:
- Biochemical and immunochemical analysis of β-adrenergic receptor adenylate cyclase complexesTrends in Pharmacological Sciences, 1982
- Antibodies raised against β-adrenergic receptors stimulate adenylate cyclaseBiochemical and Biophysical Research Communications, 1981
- Anti-alprenolol anti-idiotypic antibodies bind to beta-adrenergic receptors and modulate catecholamine-sensitive adenylate cyclase.Proceedings of the National Academy of Sciences, 1980
- Purification of the regulatory component of adenylate cyclase.Proceedings of the National Academy of Sciences, 1980
- Biochemical Properties of Hormone-Sensitive Adenylate CyclaseAnnual Review of Biochemistry, 1980
- The role of hormone receptors and GTP-regulatory proteins in membrane transductionNature, 1980
- New directions in adrenergic receptor research Part ITrends in Pharmacological Sciences, 1980
- Visualization of the turkey erythrocyte β‐adrenergic receptorJournal of Supramolecular Structure, 1980
- Affinity Chromatography of the β‐Adrenergic Receptor from Turkey ErythrocytesEuropean Journal of Biochemistry, 1979
- Isolation of adenylate cyclase-free, beta-adrenergic receptor from turkey erythrocyte membranes by affinity chromatography.Proceedings of the National Academy of Sciences, 1977