Construction of the genetic map of the polyoma genome

Abstract

Seven early mutants, 3 late mutants and 1 plaque morphology mutant of polyoma were mapped by marker rescue using wild-type restriction endonuclease fragments. The early mutants map between 1.0-26.4 units from the Eco RI site, a region previously shown to correspond to the 3''-OH terminal half of early RNA. The late mutants and the plaque morphology mutant map between 26.6-45.4 map units, a region previously shown to correspond to the 3''-OH terminal half of the late RNA. Analysis of the genotype of rescued virus demonstrated that the modification of the mutant DNA during marker rescue was limited to the region of the genome covered by the wild-type restriction endonuclease fragment tested.