Effect of cytosine arabinoside triphosphate on the incorporation of3H-thymidine in mitochondria isolated from HeLa cells
- 1 October 1979
- journal article
- research article
- Published by Wiley in Journal of Cellular Physiology
- Vol. 101 (1), 17-23
- https://doi.org/10.1002/jcp.1041010104
Abstract
HeLa cell mitochondria were allowed to incorporate 3H‐thymidine in a cell free system and the effect of ethidium bromide, cytosine arabinoside and cytosine arabinoside triphosphate on the labeling of mitochondrial DNA was studied. The labeled products, isolated by sedimentation velocity in CsCl‐ethidium bromide two‐step gradients, showed similar sedimentation profiles as in vivo labeled mtDNA. Cytosine arabinoside triphosphate and ethidium bromide strongly inhibited the labeling of mitochondrial DNA, whereas cytosine arabinoside appeared to be much less effective. Tritiated deoxycytidine was found to be incorporated by isolated mitochondria, whereas cytosine arabinoside was shown to enter the mitochondrial acid‐soluble pool but not to be incorporated in acid‐insoluble form. These results are in agreement with the previously reported findings of in vivo experiments.This publication has 14 references indexed in Scilit:
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