Differential Effects of the Perinatal Steroid Environment on Three Sexually Dimorphic Parameters of the Rat Brain1
- 1 May 1985
- journal article
- research article
- Published by Oxford University Press (OUP) in Biology of Reproduction
- Vol. 32 (4), 855-864
- https://doi.org/10.1095/biolreprod32.4.855
Abstract
An enzyme-linked immunosorbent assay (ELISA), which utilized anti-H-2 monoclonal antibody, was used to detect H-2 antigens on preimplantation mouse embryos. All embryonic stages studied, including unfertilized eggs and 1-cell, 2-cell, 8-cell, and blastocyst-stage embryos, showed the presence of H-2 antigens. To prove that the H-2 antigens were not cytophilically adsorbed to the embryos, blastocysts were treated with papain to strip off the H-2 antigens, and then the embryos were further incubated to allow the H-2 antigens to regenerate. After a 3-h incubation time, 60% of the H-2 antigens on the embryos had reappeared, proving that the H-2 antigens were synthesized by the embryos themselves.Keywords
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