DNA-Dependent RNA Polymerases of the three Orders of Methanogens

Abstract

The DNA-dependent RNA polymerases of members of the three orders of methanogens were purified and their enzymatic properties described. The enzymes consist of 7-8 polypeptides. Although these differed in molecular mass, the four heaviest components could be allied to components of the enzyme of Methanobacterium thermoautotrophicum, W by cross-reaction with antibodies directed against the denatured polypeptides of this enzyme. The antisera against native RNA polymerases isolated from representatives of the different orders, on the other hand, gave rise to serological cross-reaction between different genera but not between different families and orders. These antisera are thus useful for taxonomic purposes. The RNA polymerase of the extreme thermophile Methanothermus fervidus shows a rather low thermostability. No factors having a stabilizing influence on the enzyme could be detected.