Properties of thin‐rod immobilized pH gradients
- 1 January 1988
- journal article
- research article
- Published by Wiley in Electrophoresis
- Vol. 9 (4), 172-182
- https://doi.org/10.1002/elps.1150090405
Abstract
Immobilized pH gradient gel rods, 1.5 mm in diameter, were cast with a manifold connected to high‐precision burettes. The reproducibility of gel length was ca. 1.7 mm. The average standard deviation σx for spot position was 2 mm after one‐dimensional and 5.8 mm after two‐dimensional runs. In order to bring to completion the elution of the salt fronts into the electrode compartments, carrier ampholytes had to be included in the gel formulation at concentrations of at least 0.5–1%, depending on the pH range. The presence of carrier ampholytes, however, was troublesome in two respects: the gel tended to shrink and the cathodic bands drifted with time. Ionic components in the sample were tolerated up to the following concentrations: NaCl 8 μmoles, sodium dodecyl sulfate 10 μg per tube. In presence of non‐ionic detergents, the gels moved as a whole towards the cathode.This publication has 32 references indexed in Scilit:
- Horizontal two‐dimensional electrophoresis with immobilized pH gradients in the first dimension in the presence of nonionic detergentElectrophoresis, 1987
- Immobilized pH gradients in capillary tubes and two‐dimensional gel electrophoresisElectrophoresis, 1986
- Simplified procedure for the preparation of immobilized pH gradient gelsElectrophoresis, 1986
- Two‐dimensional analysis of membrane proteins with isoelectric focusing in immobilized pH gradients in the first dimensionElectrophoresis, 1986
- On the reproducibility of band position in electrophoretic separationsElectrophoresis, 1986
- Serum fractionation on immobilized pH gradients with one‐ and two‐dimensional techniquesElectrophoresis, 1984
- Forming reproducible density and solute gradients by computer‐controlled cooperation of stepmotor‐driven burettesElectrophoresis, 1984
- Computer-aided standardization of two-dimensional electrophoresis: Improvement of biophysical description of proteins in relativeversus absolute coordinate systemsElectrophoresis, 1984
- High resolution two‐dimensional polyacrylamide gel electrophoresis. I. Methodological proceduresElectrophoresis, 1983
- Enhancement of resolution in two‐dimensional gel electrophoresis and simultaneous resolution of acidic and basic proteinsElectrophoresis, 1982