Clones of alloreactive T cells
- 1 February 1980
- journal article
- research article
- Published by Springer Nature in Immunogenetics
- Vol. 10 (1-4), 175-184
- https://doi.org/10.1007/bf01561566
Abstract
We have developed a method which allows us to clone and reclone primed responder T cells derived from serially restimulated murine mixed lymphocyte cultures. We have derived clones from two such mixed lymphocyte cultures, A anti-B6 [A(B6)] and A anti-(B6XA)F1 [A(B6A)]. In the A (B6) system, we have isolated clones which can be stimulated by B6 but not by (B6XA)F1 cells. This implies the presence of a unique parental H-2b MLR determinant which is absent on semi-allogeneic (B6XA)F1 cells. In the A(B6A) system, we have isolated clones which can be stimulated by (B6XA)F1 cells but not by B6 cells. This confirms our previous observation on the presence of unique hybrid MLR stimulating determinants on (B6XA)F1 cells. Many of the “clones” derived primarily from soft agar seem to be contaminated and contain several different sets of primed responder cells with different reactivity patterns. Experiments in which we subcloned cells exhibiting selected reactivity patterns from such contaminated primary clones suggested that a T-cell growth factor or accessory cell is required for proliferation in soft agar following alloantigen recognition by primed responder cells.Keywords
This publication has 23 references indexed in Scilit:
- Fine specificity of a continuously growing killer cell clone specific for H‐Y antigenEuropean Journal of Immunology, 1979
- Clones of alloreactive T cellsNature, 1978
- Long term culture of tumour-specific cytotoxic T cellsNature, 1977
- Secondary cell-mediated lympholysis: importance of H-2 LD and SD factors.The Journal of Experimental Medicine, 1976
- GENERATION OF CYTOTOXIC T LYMPHOCYTES IN VITROThe Journal of Experimental Medicine, 1974
- Generation of T Memory Cells in One-Way Mixed Lymphocyte Culture.Scandinavian Journal of Immunology, 1974
- GENERATION OF CYTOTOXIC T LYMPHOCYTES IN VITROThe Journal of Experimental Medicine, 1974
- GENERATION OF CYTOTOXIC T LYMPHOCYTES IN VITROThe Journal of Experimental Medicine, 1974
- T cell synergy in mixed lymphocyte culture‐induced cytolysisEuropean Journal of Immunology, 1974
- Interaction of thymus lymphocytes with histoincompatible cells: IV. Mixed lymphocyte reactions of activated thymus lymphocytesCellular Immunology, 1974