An enzymic method for the estimation of l-tryptophan
- 1 September 1961
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 80 (3), 462-464
- https://doi.org/10.1042/bj0800462
Abstract
An enzymic method is described for the estimation of L-tryptophan in biological material, in the range 1-60 [mu]g. L-Tryptophan is converted quantitatively into indole by the enzyme tryptophanase, and the indole measured colorimetrically, after extraction with light petroleum, with either p-dimethylaminobenzaldehyde or p-dimethyl-aminocinnamic aldehyde reagents.Keywords
This publication has 4 references indexed in Scilit:
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- Der Gehalt an freiem Tryptophan und Kynurenin bei den Genotypen a+ und a von Ephestia kühniella während der EntwicklungZeitschrift für Naturforschung B, 1957
- FREE TRYPTOPHANE IN BLOOD AND URINEJournal of Biological Chemistry, 1946
- The quantitative determination of indole in bacterial culturesBiochemical Journal, 1934