Molecular cloning of an EcoRI fragment which contains 4.5S and 5S RNA genes in tobacco chloroplasts.

Abstract

A 0.9 .times. 106 dalton EcoRI fragment of tobacco chloroplast DNA was cloned in Escherichia coli plasmid pMB9. This fragment contains the chloroplast 4.5S and 5S rRNA genes. A physical map of the recombinant plasmid was constructed by cleavage with several restriction endonucleases and Southern hybridization with 32P-labeled 4.5S and 5S RNA.